Rat ANXA2 (Annexin A2) ELISA Kit | ELK5765

Rat ANXA2 (Annexin A2) ELISA Kit | ELK5765 | Gentaur UK, US & Europe Distribution

Alternative Names: ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

Species: Rat

Assay Type: Sandwich

Sensitivity: 43 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A2 (ANXA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Annexin A2 (ANXA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A2 (ANXA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A2 (ANXA2) in the samples is then determined by comparing the OD of the samples to the standard curve.