Rat ANXA5 (Annexin A5) ELISA Kit | ELK1649

Rat ANXA5 (Annexin A5) ELISA Kit | ELK1649 | Gentaur UK, US & Europe Distribution

Alternative Names: ANX5; ENX2; PP4; PAP-I; CBP-I; Anchorin CII; Calphobindin I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; Thromboplastin inhibitor; Vascular anticoagulant-alpha

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Apoptosis;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A5 (ANXA5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Annexin A5 (ANXA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A5 (ANXA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A5 (ANXA5) in the samples is then determined by comparing the OD of the samples to the standard curve.