Rat APOA4 (Apolipoprotein A4) ELISA Kit | ELK6726

Rat APOA4 (Apolipoprotein A4) ELISA Kit | ELK6726 | Gentaur UK, US & Europe Distribution

Alternative Names: ApoA-IV; ApoAIV; Apo-A4

Species: Rat

Assay Type: Sandwich

Sensitivity: 27.7 pg/mL

Standard: 50000 pg/mL

Detection Range: 781.25-50000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A4 (APOA4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein A4 (APOA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A4 (APOA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A4 (APOA4) in the samples is then determined by comparing the OD of the samples to the standard curve.