Rat Apolipoprotein C-II (APOC2) ELISA Kit | AE62894RA

Rat Apolipoprotein C-II (APOC2) ELISA Kit | AE62894RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: APOC2

Alternative Name: N/A

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.137 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤8.0%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate APOC2 in samples. An antibody specific for APOC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPOC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Apolipoprotein C2 is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Apolipoprotein C-II (apoC-II) is a necessary cofactor for the activation of lipoprotein lipase (LPL), the enzyme that hydrolyzes triglycerides in plasma and transfers the fatty acids to tissues.The APOC2 cDNA sequence encodes a deduced 79-amino acid protein. Using synthetic oligonucleotides as probes, Sakaguchi et al. (1984) isolated APOC2 from a human cDNA library.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .