Rat ARRb2 (Arrestin Beta 2) ELISA Kit | ELK8684

Rat ARRb2 (Arrestin Beta 2) ELISA Kit | ELK8684 | Gentaur UK, US & Europe Distribution

Alternative Names: ARB2; ARR2; ARR-B2; Arrestin 3; Beta Arrestin-2

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.4 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Endocrinology;Neuro science;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arrestin Beta 2 (ARRb2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arrestin Beta 2 (ARRb2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arrestin Beta 2 (ARRb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arrestin Beta 2 (ARRb2) in the samples is then determined by comparing the OD of the samples to the standard curve.