Rat Bax (Bcl2 Associated X Protein) ELISA Kit | ELK5698

Rat Bax (Bcl2 Associated X Protein) ELISA Kit | ELK5698 | Gentaur UK, US & Europe Distribution

Alternative Names: BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

Species: Rat

Assay Type: Sandwich

Sensitivity: 4.6 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Apoptosis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the OD of the samples to the standard curve.