Rat BLC1 (B-Lymphocyte Chemoattractant 1) ELISA Kit | ELK6662

Rat BLC1 (B-Lymphocyte Chemoattractant 1) ELISA Kit | ELK6662 | Gentaur UK, US & Europe Distribution

Alternative Names: CXCL13; SCYB13; BLC; BCA1; BLR1L; ANGIE; ANGIE2; Chemokine (C-X-C-Motif) ligand 13; Small Inducible Cytokine B Subfamily (Cys-X-Cys Motif) Member 13

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Immune molecule;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant 1 (BLC1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Lymphocyte Chemoattractant 1 (BLC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Chemoattractant 1 (BLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Chemoattractant 1 (BLC1) in the samples is then determined by comparing the OD of the samples to the standard curve.