Rat BNP (Brain Natriuretic Peptide) ELISA Kit | ELK2590

Rat BNP (Brain Natriuretic Peptide) ELISA Kit | ELK2590 | Gentaur UK, US & Europe Distribution

Alternative Names: GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 1.77 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Cardiovascular biology;Neuro science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Brain Natriuretic Peptide (BNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Brain Natriuretic Peptide (BNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Natriuretic Peptide (BNP) in the samples is then determined by comparing the OD of the samples to the standard curve.