Rat C-C motif chemokine 6 (CCL6) ELISA Kit | AE62814RA

Rat C-C motif chemokine 6 (CCL6) ELISA Kit | AE62814RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: CCL6

Alternative Name: Small-inducible cytokine A6

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤3.4%

Inter-Assay: ≤10.6%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CCL6 in samples. An antibody specific for CCL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: C10, known also as SCYA6 (small inducible cytokine A6) is one of several mRNAs that are acutely expressed in myelopoietic mouse bone marrow cultures stimulated by GM-CSF. It is also strongly elevated during the induction of neutrophilic differentiation of 32D cells that are dependent on the presence of IL3 or G-CSF.C10 is related to MIP-1. Its protein sequence demonstrates that C10 belongs to the family of chemotactic cytokines known as Chemokines (beta-Chemokines or CC-Chemokines) . The protein contains six instead of four conserved cysteine residues. The C10 gene contains a second exon not found in other Chemokines. This exon encodes a 16 amino acid sequence, which is inserted into the amino terminal region of the protein and plays a key role in recognizing or activating chemokine receptors.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .