Rat C1INH (Complement 1 Inhibitor) ELISA Kit | ELK5547

Rat C1INH (Complement 1 Inhibitor) ELISA Kit | ELK5547 | Gentaur UK, US & Europe Distribution

Alternative Names: SERPING1; C1IN; C1-INH; C1NH; HAE1; HAE2; Serpin Peptidase Inhibitor Clade G Member 1; Angioedema Hereditary; C1 Inhibitor; C1 Esterase Inhibitor

Species: Rat

Assay Type: Sandwich

Sensitivity: 2.61 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the OD of the samples to the standard curve.