Rat C3a (Complement Component 3a) ELISA Kit | ELK1591

Rat C3a (Complement Component 3a) ELISA Kit | ELK1591 | Gentaur UK, US & Europe Distribution

Alternative Names: Complement Component 3a

Species: Rat

Assay Type: Sandwich

Sensitivity: 2.81 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3a (C3a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Component 3a (C3a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3a (C3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 3a (C3a) in the samples is then determined by comparing the OD of the samples to the standard curve.