Rat CALB (Calbindin) ELISA Kit | ELK6401

Rat CALB (Calbindin) ELISA Kit | ELK6401 | Gentaur UK, US & Europe Distribution

Alternative Names: CAB27; Calbindin 1, 28kDa; Calbindin D28; Vitamin D-dependent calcium-binding protein, avian-type

Species: Rat

Assay Type: Sandwich

Sensitivity: 3.3 pg/mL

Standard: 600 pg/mL

Detection Range: 9.38-600 pg/mL

Sample Type: Serum, plasma, tissue homogenates, urine and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin (CALB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calbindin (CALB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calbindin (CALB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calbindin (CALB) in the samples is then determined by comparing the OD of the samples to the standard curve.