Rat CAR (Constitutive Androstane Receptor) ELISA Kit | ELK7571

Rat CAR (Constitutive Androstane Receptor) ELISA Kit | ELK7571 | Gentaur UK, US & Europe Distribution

Alternative Names: NR1I3; NR1-I3; CAR1; MB67; Nuclear Receptor Subfamily 1, Group I, Member 3; Constitutive active response; Constitutive activator of retinoid response; Orphan nuclear receptor MB67

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Constitutive Androstane Receptor (CAR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Constitutive Androstane Receptor (CAR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Constitutive Androstane Receptor (CAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Constitutive Androstane Receptor (CAR) in the samples is then determined by comparing the OD of the samples to the standard curve.