749

Rat Carbonic anhydrase 2 (CA2) ELISA Kit | KTE100849

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SKU:
749-KTE100849
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Description

Rat Carbonic anhydrase 2 (CA2) ELISA Kit | KTE100849 | Gentaur UK, US & Europe Distribution

Application: This Rat Carbonic anhydrase 2 (CA2) ELISA Kit employs a two-site sandwich ELISA to quantitate CA2 in samples. An antibody specific for CA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Carbonic anhydrase 2 microplate
• Rat Carbonic anhydrase 2 standard
• Rat Carbonic anhydrase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Carbonic anhydrase 2 (CA2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CA2. No significant cross-reactivity or interference between Rat CA2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Carbonic anhydrase II (gene name CA2), is one of fourteen forms of humanαcarbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II is expressed at high levels in osteoclasts during bone resorption. Laitala and Vaananen (1994) demonstrated in 2 in vitro culture systems that antisense RNA and DNA blocked expression of CA II and led to decreased bone resorption. In the choroid plexus, carbonic anhydrase II supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.

Alternative Names: CA2; CA-II; CAII; Car2; carbonate dehydratase; carbonic anhydrase B; carbonic dehydratase

Search name: CA2; CA-II; CAII; Car2; carbonate dehydratase; carbonic anhydrase B; carbonic dehydratase

Tag: CA2

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