Rat CASP1 (Caspase 1) ELISA Kit | ELK6099

Rat CASP1 (Caspase 1) ELISA Kit | ELK6099 | Gentaur UK, US & Europe Distribution

Alternative Names: ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.112 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 1 (CASP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caspase 1 (CASP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 1 (CASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 1 (CASP1) in the samples is then determined by comparing the OD of the samples to the standard curve.