Rat CD3 (Cluster of Differentiation 3) ELISA Kit | ELK9162

Rat CD3 (Cluster of Differentiation 3) ELISA Kit | ELK9162 | Gentaur UK, US & Europe Distribution

Alternative Names: CD3; CD3e antigen; CD3e antigen; epsilon polypeptide; TiT3 complex; CD3e molecule; epsilon; CD3-TCR complex; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex; epsilon subunit of T3; T-cell surface antigen T3; Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Immunology; Signal Transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster of Differentiation 3 (CD3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cluster of Differentiation 3 (CD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster of Differentiation 3 (CD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster of Differentiation 3 (CD3) in the samples is then determined by comparing the OD of the samples to the standard curve.