Rat CD34 (Cluster Of Differentiation 34) ELISA Kit | ELK8913

Rat CD34 (Cluster Of Differentiation 34) ELISA Kit | ELK8913 | Gentaur UK, US & Europe Distribution

Alternative Names: Hematopoietic progenitor cell antigen CD34

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.37 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Developmental science;Genetic science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 34 (CD34) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cluster Of Differentiation 34 (CD34) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 34 (CD34), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 34 (CD34) in the samples is then determined by comparing the OD of the samples to the standard curve.