Rat CDHE (Cadherin, Epithelial) ELISA Kit | ELK8615

Rat CDHE (Cadherin, Epithelial) ELISA Kit | ELK8615 | Gentaur UK, US & Europe Distribution

Alternative Names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule

Species: Rat

Assay Type: Sandwich

Sensitivity: 25.1 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: serum, plasma, urine, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin, Epithelial (CDHE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cadherin, Epithelial (CDHE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin, Epithelial (CDHE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin, Epithelial (CDHE) in the samples is then determined by comparing the OD of the samples to the standard curve.