Rat Cellular tumor antigen p53 (TP53) ELISA Kit | KTE100044

Rat Cellular tumor antigen p53 (TP53) ELISA Kit | KTE100044 | Gentaur UK, US & Europe Distribution

Application: This Rat Cellular tumor antigen p53 (TP53) ELISA Kit employs a two-site sandwich ELISA to quantitate TP53 in samples. An antibody specific for TP53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTP53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Cellular tumor antigen p53 microplate
• Rat Cellular tumor antigen p53 standard
• Rat Cellular tumor antigen p53 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Cellular tumor antigen p53 (TP53) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TP53. No significant cross-reactivity or interference between Rat TP53 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity.

Alternative Names: TP53; FLJ92943; LFS1; TRP53; p53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53

Search name: TP53; FLJ92943; LFS1; TRP53; p53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53

Tag: TP53