Rat CELSR2 (Cadherin EGF LAG Seven Pass G-Type Receptor 2) ELISA Kit | ELK6690

Rat CELSR2 (Cadherin EGF LAG Seven Pass G-Type Receptor 2) ELISA Kit | ELK6690 | Gentaur UK, US & Europe Distribution

Alternative Names: CDHF10; EGFL2; Flamingo 1; MEGF3; Cadherin family member 10; Epidermal growth factor-like protein 2; Multiple epidermal growth factor-like domains protein 3

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.24 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) in the samples is then determined by comparing the OD of the samples to the standard curve.