Rat CFB (Complement Factor B) ELISA Kit | ELK5823

Rat CFB (Complement Factor B) ELISA Kit | ELK5823 | Gentaur UK, US & Europe Distribution

Alternative Names: CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein

Species: Rat

Assay Type: Sandwich

Sensitivity: 2.81 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Hematology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor B (CFB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Factor B (CFB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor B (CFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor B (CFB) in the samples is then determined by comparing the OD of the samples to the standard curve.