Rat CFH (Complement Factor H) ELISA Kit | ELK1721

Rat CFH (Complement Factor H) ELISA Kit | ELK1721 | Gentaur UK, US & Europe Distribution

Alternative Names: CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 5.77 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Metabolic pathway;Infection immunity;Immune molecule

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Complement Factor H (CFH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Factor H (CFH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the OD of the samples to the standard curve.