Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) ELISA Kit | ELK6704

Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) ELISA Kit | ELK6704 | Gentaur UK, US & Europe Distribution

Alternative Names: CLC; NR6; BSF3; CISS2; NNT1; Novel Neurotrophin-1; B Cell-Stimulating Factor-3

Species: Rat

Assay Type: Sandwich

Sensitivity: 12.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Cardiovascular biology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cardiotrophin Like Cytokine Factor 1 (CLCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiotrophin Like Cytokine Factor 1 (CLCF1) in the samples is then determined by comparing the OD of the samples to the standard curve.