Rat COL2 (Collagen Type II) ELISA Kit | ELK2620

Rat COL2 (Collagen Type II) ELISA Kit | ELK2620 | Gentaur UK, US & Europe Distribution

Alternative Names: Collagen Type 2; Col 2; Collagen Type II; Col II

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.29 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Developmental science;Hepatology;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II (COL2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type II (COL2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II (COL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type II (COL2) in the samples is then determined by comparing the OD of the samples to the standard curve.