Rat COL3a1 (Collagen Type III Alpha 1) ELISA Kit | ELK6514

Rat COL3a1 (Collagen Type III Alpha 1) ELISA Kit | ELK6514 | Gentaur UK, US & Europe Distribution

Alternative Names: COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1 (III) chain

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.277 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type III Alpha 1 (COL3a1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type III Alpha 1 (COL3a1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type III Alpha 1 (COL3a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type III Alpha 1 (COL3a1) in the samples is then determined by comparing the OD of the samples to the standard curve.