Rat CTN (Creatinine) ELISA Kit | ELK9274

Rat CTN (Creatinine) ELISA Kit | ELK9274 | Gentaur UK, US & Europe Distribution

Alternative Names: Creatinine

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.89 µg/mL

Standard: 200 µg/mL

Detection Range: 3.13-200 µg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Creatinine (CTN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Creatinine (CTN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Creatinine (CTN) in the samples is then determined by comparing the OD of the samples to the standard curve.