Rat Cyclooxygenase-1 (COX-1) ELISA Kit | KTE100391

Rat Cyclooxygenase-1 (COX-1) ELISA Kit | KTE100391 | Gentaur UK, US & Europe Distribution

Application: This Rat Cyclooxygenase-1 (COX-1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGS1 in samples. An antibody specific for PTGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Cyclooxygenase-1 microplate
• Rat Cyclooxygenase-1 standard
• Rat Cyclooxygenase-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Cyclooxygenase-1 (COX-1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTGS1. No significant cross-reactivity or interference between Rat PTGS1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Recent research has shown that the inhibition of COX-1 is the main reason why aspirin is effective at reducing cardiac events (as opposed to aspirin's anti-platelet aggregation effects) . PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants called COX-1 and COX-3. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors.

Alternative Names: PTGS1; RP11-542K23.6; COX1; COX3; PCOX1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 1

Search name: PTGS1; RP11-542K23.6; COX1; COX3; PCOX1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 1

Tag: PTGS1