Rat CYP11B1 (Cytochrome P450 11B1) ELISA Kit | ELK6641

Rat CYP11B1 (Cytochrome P450 11B1) ELISA Kit | ELK6641 | Gentaur UK, US & Europe Distribution

Alternative Names: CPN1; CYP11B; FHI; CYPXIB1; S11BH; 11-Beta-Hydroxylase; Cytochrome P450 Family 11 Subfamily B Polypeptide 1; Steroid 11-beta-hydroxylase; Cytochrome P-450c11

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.27 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 11B1 (CYP11B1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 11B1 (CYP11B1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 11B1 (CYP11B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 11B1 (CYP11B1) in the samples is then determined by comparing the OD of the samples to the standard curve.