Rat DAO (Diamine Oxidase) ELISA Kit | ELK2230

Rat DAO (Diamine Oxidase) ELISA Kit | ELK2230 | Gentaur UK, US & Europe Distribution

Alternative Names: AOC1; ABP1; KAO; Histaminase; Kidney amine oxidase; Amiloride Binding Protein 1; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.236 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diamine Oxidase (DAO) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diamine Oxidase (DAO) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diamine Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diamine Oxidase (DAO) in the samples is then determined by comparing the OD of the samples to the standard curve.