Rat DCD (Dermcidin) ELISA Kit | ELK6013

Rat DCD (Dermcidin) ELISA Kit | ELK6013 | Gentaur UK, US & Europe Distribution

Alternative Names: HCAP; AIDD; DCD1; DSEP; PIF; Proteolysis Inducing Factor; Preproteolysin; Diffusible Survival/Evasion Peptide; Survival Promoting Peptide

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and sweat.

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermcidin (DCD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dermcidin (DCD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermcidin (DCD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dermcidin (DCD) in the samples is then determined by comparing the OD of the samples to the standard curve.