Rat Disks large homolog 3 (DLG3) ELISA Kit | KTE100754

Rat Disks large homolog 3 (DLG3) ELISA Kit | KTE100754 | Gentaur UK, US & Europe Distribution

Application: This Rat Disks large homolog 3 (DLG3) ELISA Kit employs a two-site sandwich ELISA to quantitate DLG3 in samples. An antibody specific for DLG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLG3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Disks large homolog 3 microplate
• Rat Disks large homolog 3 standard
• Rat Disks large homolog 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Disks large homolog 3 (DLG3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLG3. No significant cross-reactivity or interference between Rat DLG3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DLG3 is a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation. Alternatively spliced transcript variants have been described. The predicted 817-amino acid protein contains the 3 DHR segments, central SH3 motif, and C-terminal guanylate kinase domain characteristic of MAGUK proteins. NEDLG shares 75% and 60% protein sequence identity with DLG1 and Drosophila dlg, respectively. NEDLG is highly expressed in neuronal and endocrine tissues.

Alternative Names: DLG3; RP11-291O7.3; KIAA1232; MRX; MRX90; NEDLG; SAP102; XLMR; neuroendocrine-DLG; synapse-associated protein 102

Search name: DLG3; RP11-291O7.3; KIAA1232; MRX; MRX90; NEDLG; SAP102; XLMR; neuroendocrine-DLG; synapse-associated protein 102

Tag: DLG3