Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit | KTE100871

Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit | KTE100871 | Gentaur UK, US & Europe Distribution

Application: This Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2E in samples. An antibody specific for POLR2E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2E bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 microplate
• Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 standard
• Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POLR2E. No significant cross-reactivity or interference between Rat POLR2E and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. A second site of hybridization to 11q23.1 was thought to represent a pseudogene, most likely a retroposon, since no remnants of introns could be identified in the study of genomic DNA.

Alternative Names: POLR2E; RPABC1; RPB5; XAP4; hRPB25; hsRPB5; DNA directed RNA polymerase 23 kda polypeptide; DNA directed RNA polymerase polypeptide E

Search name: POLR2E; RPABC1; RPB5; XAP4; hRPB25; hsRPB5; DNA directed RNA polymerase 23 kda polypeptide; DNA directed RNA polymerase polypeptide E

Tag: POLR2E