Rat Doublesex- and mab-3-related transcription factor C1 (DMRTC1) ELISA Kit | KTE100744

Rat Doublesex- and mab-3-related transcription factor C1 (DMRTC1) ELISA Kit | KTE100744 | Gentaur UK, US & Europe Distribution

Application: This Rat Doublesex- and mab-3-related transcription factor C1 (DMRTC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRTC1 in samples. An antibody specific for DMRTC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Doublesex- and mab-3-related transcription factor C1 microplate
• Rat Doublesex- and mab-3-related transcription factor C1 standard
• Rat Doublesex- and mab-3-related transcription factor C1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Doublesex- and mab-3-related transcription factor C1 (DMRTC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DMRTC1. No significant cross-reactivity or interference between Rat DMRTC1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DMRTC1 (DMRT-like family C1) is a 182 amino acid nuclear protein that exists as three alternatively spliced isoforms and belongs to the DMRT family. Involved in sexual development, DMRTC1 is expressed in Sertoli cells in male testis but, unlike other DMRT family members, it does not contain a DM DNA-binding domain. Highly expressed in ovary and testis. Detected in brain and in many other tissues. The gene encoding DMRTC1 maps to the human X chromosome, which consists of about 153 million base pairs and nearly 1, 000 genes. The combination of an X and Y chromosome lead to normal male development while two copies of X lead to normal female development. There are a number of conditions related to an unsual number and combination of sex chromosomes being inherited, including Klinefelter’s syndrome, Turner’s syndrome, and Triple X syndrome.

Alternative Names: DMRTC1; RP11-69A21.1; doublesex-mab-3

Search name: DMRTC1; RP11-69A21.1; doublesex-mab-3

Tag: DMRTC1