855

Rat Dyn (Big Dynorphin) ELISA Kit | ELK5766

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SKU:
855-ELK5766-GEN
NULL588.00 - NULL704.00

Description

Rat Dyn (Big Dynorphin) ELISA Kit | ELK5766 | Gentaur UK, US & Europe Distribution

Alternative Names: B-DYN; Biocytin 13 Dynorphin A 1-13 Amide

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 1.42 pg/mL

Standard: 300 pg/mL

Detection Range: 4.69-300 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Signal transduction;Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Big Dynorphin (Dyn) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Big Dynorphin (Dyn) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Big Dynorphin (Dyn) in the samples is then determined by comparing the OD of the samples to the standard curve.

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