Rat ELA2 (Elastase 2, Neutrophil) ELISA Kit | ELK1521

Rat ELA2 (Elastase 2, Neutrophil) ELISA Kit | ELK1521 | Gentaur UK, US & Europe Distribution

Alternative Names: ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.64 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2, Neutrophil (ELA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Elastase 2, Neutrophil (ELA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2, Neutrophil (ELA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 2, Neutrophil (ELA2) in the samples is then determined by comparing the OD of the samples to the standard curve.