Rat ERa (Estrogen Receptor Alpha) ELISA Kit | ELK5627

Rat ERa (Estrogen Receptor Alpha) ELISA Kit | ELK5627 | Gentaur UK, US & Europe Distribution

Alternative Names: ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3, Group A, Member 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.126 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Alpha (ERa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrogen Receptor Alpha (ERa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Alpha (ERa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Alpha (ERa) in the samples is then determined by comparing the OD of the samples to the standard curve.