Rat F2 (Coagulation Factor II) ELISA Kit | ELK1517

Rat F2 (Coagulation Factor II) ELISA Kit | ELK1517 | Gentaur UK, US & Europe Distribution

Alternative Names: FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2

Species: Rat

Assay Type: Sandwich

Sensitivity: 3.5 ng/mL

Standard: 500 ng/mL

Detection Range: 7.82-500 ng/mL

Sample Type: Serum

Assay Lenght: 3.5h

Research Area: Signal transduction;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor II (F2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor II (F2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor II (F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor II (F2) in the samples is then determined by comparing the OD of the samples to the standard curve.