Description
Rat F6 (Coagulation Factor VI) ELISA Kit | ELK3627 | Gentaur UK, US & Europe Distribution
Alternative Names: Coagulation Factor Va
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.24 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Plasma.
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VI (F6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor VI (F6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VI (F6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VI (F6) in the samples is then determined by comparing the OD of the samples to the standard curve.