Rat F7 (Coagulation Factor VII) ELISA Kit | ELK6386

Rat F7 (Coagulation Factor VII) ELISA Kit | ELK6386 | Gentaur UK, US & Europe Distribution

Alternative Names: FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa

Species: Rat

Assay Type: Sandwich

Sensitivity: 12.4 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Plasma

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VII (F7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor VII (F7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VII (F7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VII (F7) in the samples is then determined by comparing the OD of the samples to the standard curve.