Rat FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit | ELK2598

Rat FGF2 (Fibroblast Growth Factor 2, Basic) ELISA Kit | ELK2598 | Gentaur UK, US & Europe Distribution

Alternative Names: B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 4.88 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fibroblast Growth Factor 2, Basic (FGF2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the samples is then determined by comparing the OD of the samples to the standard curve.