Rat FOS (V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit | ELK5685

Rat FOS (V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit | ELK5685 | Gentaur UK, US & Europe Distribution

Alternative Names: Cfos; C-Fos; G0S7; Proto-oncogene c-Fos; G0/G1 Switch Regulatory Protein 7; Cellular oncogene fos

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.06 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) in the samples is then determined by comparing the OD of the samples to the standard curve.