Rat G protein-activated inward rectifier potassium channel 4 (KCNJ5) ELISA Kit | KTE100728

Rat G protein-activated inward rectifier potassium channel 4 (KCNJ5) ELISA Kit | KTE100728 | Gentaur UK, US & Europe Distribution

Application: This Rat G protein-activated inward rectifier potassium channel 4 (KCNJ5) ELISA Kit employs a two-site sandwich ELISA to quantitate KCNJ5 in samples. An antibody specific for KCNJ5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNJ5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNJ5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNJ5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat G protein-activated inward rectifier potassium channel 4 microplate
• Rat G protein-activated inward rectifier potassium channel 4 standard
• Rat G protein-activated inward rectifier potassium channel 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat G protein-activated inward rectifier potassium channel 4 (KCNJ5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KCNJ5. No significant cross-reactivity or interference between Rat KCNJ5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KCNJ5 is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. It may associate with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. Girk4 associated with Girk1 during or shortly after subunit synthesis and allowed appropriate glycosylation of the Girk1 subunit to a form seen in native atrial tissue. The C terminus of Girk4 was required for cell surface localization. Girk1 appeared intracellular in atrial myocytes isolated from Girk4-knockout mice, and it was not maturely glycosylated, supporting an essential role for GIRK4 in processing and cell surface localization of GIRK1/GIRK4 channels in vivo.

Alternative Names: KCNJ5; CIR; GIRK4; KATP1; KIR3.4; G protein-activated inward rectifier potassium channel 4 (GIRK4) (Potassium channel; inwardly rectifying; subfamily J; member 5) (Inward rectifier K+ channel Kir3.4) (Hear

Search name: KCNJ5; CIR; GIRK4; KATP1; KIR3.4; G protein-activated inward rectifier potassium channel 4 (GIRK4) (Potassium channel; inwardly rectifying; subfamily J; member 5) (Inward rectifier K+ channel Kir3.4) (Hear

Tag: KCNJ5