Rat GAS6 (Growth Arrest Specific Protein 6) ELISA Kit | ELK4474

Rat GAS6 (Growth Arrest Specific Protein 6) ELISA Kit | ELK4474 | Gentaur UK, US & Europe Distribution

Alternative Names: AXLLG; AXSF; AXL Stimulatory Factor; AXL receptor tyrosine kinase ligand

Species: Rat

Assay Type: Sandwich

Sensitivity: 33 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest Specific Protein 6 (GAS6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Growth Arrest Specific Protein 6 (GAS6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest Specific Protein 6 (GAS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest Specific Protein 6 (GAS6) in the samples is then determined by comparing the OD of the samples to the standard curve.