Rat Gastric inhibitory polypeptide (GIP) ELISA Kit | AE58667RA

Rat Gastric inhibitory polypeptide (GIP) ELISA Kit | AE58667RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: GIP

Alternative Name: Glucose-dependent insulinotropic polypeptide

Application: ELISA

Range: 61.7-5000 pg/mL

Sensitivity: 27.1 pg/mL

Intra-Assay: ≤5.2%

Inter-Assay: ≤7.6%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate GIP in samples. An antibody specific for GIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Gastric inhibitory polypeptide (GIP), is a member of the secretin family of hormones.GIP is derived from a 153-amino acid proprotein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide. It is synthesized by K cells, which are found in the mucosa of the duodenum and the jejunum of the gastrointestinal tract.Like all endocrine hormones, it is transported by blood. It is now believed that the function of GIP is to induce insulin secretion, which is primarily stimulated by hyperosmolarity of glucose in the duodenum. After this discovery, some researchers prefer the new name of glucose-dependent insulinotropic peptide, while retaining the acronym "GIP." The amount of insulin secreted is greater when glucose is administered orally than intravenously.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .