Rat GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit | ELK6975

Rat GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit | ELK6975 | Gentaur UK, US & Europe Distribution

Alternative Names: GLCLR; GECS; GCL; Gamma Glutamylcysteine Synthetase; Glutamate Cysteine Ligase Regulatory; Gamma-ECS regulatory subunit; Glutamate cysteine ligase modifier subunit

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.052 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Modifier Subunit (GCLM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Cysteine Ligase, Modifier Subunit (GCLM) in the samples is then determined by comparing the OD of the samples to the standard curve.