Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit | ELK1581

Rat GIP (Gastric Inhibitory Polypeptide) ELISA Kit | ELK1581 | Gentaur UK, US & Europe Distribution

Alternative Names: Incretin hormone; Glucose Dependent Insulinotropic Peptide

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 27.1 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Gastroenterology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastric Inhibitory Polypeptide (GIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastric Inhibitory Polypeptide (GIP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastric Inhibitory Polypeptide (GIP) in the samples is then determined by comparing the OD of the samples to the standard curve.