Rat Glucoprotein 130 (GP130) ELISA Kit | KTE100887

Rat Glucoprotein 130 (GP130) ELISA Kit | KTE100887 | Gentaur UK, US & Europe Distribution

Application: This Rat Glucoprotein 130 (GP130) ELISA Kit employs a two-site sandwich ELISA to quantitate IL6ST in samples. An antibody specific for IL6ST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL6ST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6ST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6ST bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Glucoprotein 130 microplate
• Rat Glucoprotein 130 standard
• Rat Glucoprotein 130 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Glucoprotein 130 (GP130) ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL6ST. No significant cross-reactivity or interference between Rat IL6ST and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Glycoprotein 130 is a transmembrane protein which is the founding member of the class of all cytokine receptors. It forms one subunit of type I cytokine receptors within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand. Structurally, gp130 is composed of five fibronectin type-III domains and one immunoglobulin-like C2-type (immunoglobulin-like) domain in its extracellular portion.

Alternative Names: IL6ST; CD130; CDw130; GP130; GP130-RAPS; IL6R-beta; CD130 antigen; IL6ST nirs variant 3; OTTHUMP00000122545; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130 transducer chain; int

Search name: IL6ST; CD130; CDw130; GP130; GP130-RAPS; IL6R-beta; CD130 antigen; IL6ST nirs variant 3; OTTHUMP00000122545; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130 transducer chain; int

Tag: IL6ST