Rat gp130 (Glycoprotein 130) ELISA Kit | ELK2496

Rat gp130 (Glycoprotein 130) ELISA Kit | ELK2496 | Gentaur UK, US & Europe Distribution

Alternative Names: CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6RB; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.244 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 130 (gp130) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycoprotein 130 (gp130) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein 130 (gp130), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein 130 (gp130) in the samples is then determined by comparing the OD of the samples to the standard curve.