Rat GP39 (Glycoprotein 39, Cartilage) ELISA Kit | ELK6937

Rat GP39 (Glycoprotein 39, Cartilage) ELISA Kit | ELK6937 | Gentaur UK, US & Europe Distribution

Alternative Names: CHI3L1; CHI3-L1; YKL40; Chondrex; Chitinase 3-Like 1; Chondrocyte Protein YKL40; 39 kDa synovial protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.28 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Bone metabolism;Rheumatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 39, Cartilage (GP39) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycoprotein 39, Cartilage (GP39) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein 39, Cartilage (GP39), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein 39, Cartilage (GP39) in the samples is then determined by comparing the OD of the samples to the standard curve.