Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit | ELK2222

Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit | ELK2222 | Gentaur UK, US & Europe Distribution

Alternative Names: GSHPX1; Cellular glutathione peroxidase

Species: Rat

Assay Type: Sandwich

Sensitivity: 7.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the OD of the samples to the standard curve.